2 Questions 8 Answers 0 Followers
Questions related from Xue-hai Liang
Does anyone have any experience to do point mutation using CRISPR-CAS9 system in hNSC? How many plasmids, ssoDNA do you use for transfection. How to do the transfection with a high efficacy? How...
01 January 2015 8,360 2 View
I use dispase to dissociate ips cells, and put it about 1h in the 37oc incubator. the cells are still attached in the bottom of the plate even pipette the cells up and down several times. Is...
09 September 2014 7,817 4 View
