Xiu-Wen Kang

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Questions related from Xiu-Wen Kang

When do we need to add PMSF during sonication / lysis steps in protein purification? and why do some proteins do not add PMSF in their purification?

Base on my understanding, PMSF is an inhibiror for serine protease. I notices that some proteins (eg. ana Fld) requires PMSF in its purification process, usually during the sonication step. Other...

10 October 2019 6,498 3 View

Why isn't my myoglobin bind onto DE-52 column?

Hi everyone, The DE-52 column is equilibrated with [pH 8.0 20 mM Tris-HCl, 1 mM EDTA] buffer, and the pH is around 8.5 at 4 deg (checked with pH meter). The Mb (pI ~ 7) is dialysed overnight...

09 September 2019 7,641 10 View