Xin Lu

4 Questions 12 Answers 0 Followers

Questions related from Xin Lu

How reliably can we estimate the copy number of DNA based on amplicon sequencing?

By whole exome or whole genomic sequencing, we can estimate copy number by comparing sequencing depth of a given DNA region vs. that from a pool of normal samples, but for amplicon sequencing, how...

12 December 2013 5,584 1 View

DNA methylation - NGS vs microarray - What are the benefits?

What is the benefit of using NGS vs microarray technique for DNA methylation? Seems current methylation arrays, e.g. infinium humanmethylation450 used by TCGA, already provided very high density...

10 October 2012 1,158 5 View

What is the benefit of running next generation sequencing (NGS) for pooled short hairpin RNA (shRNA) screening?

There are lots of efforts to use NGS rather than microarray for pooled shRNA screening, my questions: Is there any unique value that NGS can provide us in this type of study that is not available...

09 September 2012 3,945 7 View

RNAseq of selected genes out of small amount of material?

Hi, are there any suggestions or references to run RNAseq for measuring expression level of only selected genes out of very small amount of material? I know there are efforts to sequence even...

09 September 2012 7,112 10 View