Xin Huang

2 Questions 2 Answers 0 Followers

Questions related from Xin Huang

How to do T cell suppression assay (mouse) ?

Hi, everyone! I tried to do mouse MDSC and T cell coculture assay, but failed. I have some questions. 1.Bone marrow derived MDSC can inhibit non-specific activated CD8+ T cell or not? (CD3 and...

10 October 2019 786 1 View

How to Prepare a Single-Cell Suspension from Mouse Spleen?

Hello, everyone ! I am trying to isolate CD8+ T cell from mouse spleen. I used CD8a microbeads (Miltenyi, 130-117-044), but the percentage of CD8+ T cell was only 60%. I used Flow cytometry to...

07 July 2019 1,901 6 View