Why cannot my FH primary antibody recognize the exogenous expressed Flag-tagged FH protein from 293T cells?

I overexpressed Flag-tagged FH (fumarase) by transfected pcDNA plasmid in 293T cells. After 3 days, I harvested the cell and extracted the whole protein. Then, I performed WB to test transfection...

16 January 2021 8,322 0 View