Can the fresh tissue be put into -80℃ first and then into 4% PFA after some time if I want to use it for immunochemstry or immunofluorescence?

I put the monkeys' brains into -80℃ to keep but I want to use them for immunochemistry and immunofluorescence. I don't know if they can stilled be used by putting into 4% PFA after frozen. Will...

01 September 2017 3,727 9 View