Xiaoqin Li

2 Questions 5 Answers 0 Followers

Questions related from Xiaoqin Li

How to gain exosomes efficiently?

When I isolate MSC-derived exosomes using differential ultracentrifugation, I finally suspend the pellets in PBS. I pipetted the pellets up and down again and again until they almost dissolved in...

22 March 2021 3,019 9 View

Is it Ok if I skip the first two centrifugation steps(300g for 10min and 2000g for 10min) when isolating the exosomes?

For exosome extraction, differential centrifugation is widely used. But what if I directly start with 10,000g for 30min, then perform the ultracentrifugation to isolate exosomes? Would it have any...

23 December 2020 9,726 8 View