Xiaoqiang Sheng

4 Questions 1 Answers 0 Followers

Questions related from Xiaoqiang Sheng

Mitochondrial identification in mouse oocyte transmission electron microscopy?

I'm new to TEM, I have some difficulties in mitochondrial identification. I am not sure which is mitochondria. Can anyone help me?

06 June 2018 3,903 0 View

Can anyone give me some advice on mitochondrial preparation for microinjection?

I am trying to do somework about mitochondria injection, facing lots of difficulties about mitochondria preparation. I prepare the mitochondria using the method from the article of Frezza et al....

12 December 2017 6,434 1 View

The method to determine the mtDNA copy number of oocyte?

I want to determine the mtDNA copy number of oocyte using qPCR, I used ten oocyte one group, the result was disappointing. Did anybody know how many oocyte do I need at least? I used REPLI-g...

09 September 2017 5,746 0 View

Can anybody offer some methods for sorting good granulosa cells?

I am trying to culture the granulosa cell from mouse. When I use the JC-1 to detect its' mitochondrial membrane potential, I can see two different populations, one has low MMP, while another has...

07 July 2017 5,823 1 View