Xiaoguang Li

2 Questions 10 Answers 0 Followers

Questions related from Xiaoguang Li

Can I use an IB antibody for IP?

Can I denature the antigen source, such as cell lysate (RIPA), and then use the IB (immunoblotting) antibody as IP (immunoprecipitation) antibody to precipitate target protein from cell lysate?

08 August 2013 4,462 16 View

What method do you suggest to remove the glycan from glycoprotein, but not destroy the protein?

I plan to remove all glycans from a 250KD glycoproteins and then use the deglycosylated protein as an antigen to do western blot . In fact, I do not have this purified glycoprotein. My material...

06 June 2013 4,270 11 View