Xiao Yi

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Questions related from Xiao Yi

Problem with genomic integration of haploid budding yeast using PCR product?

I have been trying to knock-in a designed genetic fragment at TRP1 locus taking advantage of 5FAA counter-selection (which selects for the loss of TRP1). I used PCR product as the insert,...

23 September 2016 1,863 3 View

What are the bottlenecks for developing therapeutic monoclonal antibodies?

I am a trained evolutionary biologist and now want to shift gear to biotech doing applied projects. Therapeutic monoclonal antibody development attracts my attention because antibody maturation is...

21 April 2016 9,224 3 View

Are engineered mammalian cell lines stable for extended propagation?

I want to engineer a genetic circuit to a chromosome of a mammalian cell line and want the function of the circuit to be stably expressed across extended period of cell culturing (>100...

05 April 2016 5,449 3 View

Is there anyway to expermentally control the ploidy of baker's yeast ( haploid or diploid)?

I am going to grow yeast cells in the lab for extended period of time spanning many generations. I want the population to experience haploid and diploid state in a synchronized and controlled...

08 March 2016 4,671 3 View

Why is replication-defective virus NOT used for COVID-19 vaccine?

Based on the principles of immunology, isn't true that replication-defective virus the best way for vaccination? Because 1) It is as safe as recombinant vaccines as such RNA vaccine, AAV vaccine...

01 January 1970 9,737 4 View