Xianyuan Xiang

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Questions related from Xianyuan Xiang

What is the normal morphology of primary microglia from mixed glial culture?

Hi. I used mixed glial culture, then shake the primary microglia off and replate them in 96 well plate for functional assay. I saw different morphology between WT cell and gene A knockout cell. I...

09 November 2015 9,838 7 View

How might one separate microglia from mixed glial culture and avoid debris?

I used P2 pups to prepare mixed glial culture (1 pup / flask), culture in MEM+L-glutamine, sodium pyruvate, 0.6% v/v D-(+)-glucose, P/S, 4% FBS) 6%Horse Serum for 10 or 14 days. I can see some...

26 August 2015 7,913 9 View