Xiangnan Guan

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Questions related from Xiangnan Guan

In vitro cultured mouse spleen CD3 cells activated with CD3/CD28 Abs, how will the cells differentiate in terms of CD44 and CD62?

I am new to immunology and T cell biology. I know that in vivo T cells have different subsets based on CD44 and CD62 expression, e.g. memory T cells express CD44. How about in vitro cultured T...

10 February 2018 9,161 3 View

Can someone help me with in vitro functional analysis of exhausted CD8+ T cells?

PD-1/PDL-1 blockage restores functions to exhausted CD8+ T cells. In the literature, I always see that people isolate CD8+ T cells with high-PD-1 expression, characteristic of exhaustion. These...

23 February 2015 8,487 4 View

Can anyone explain the debris/junk in my MEF cell culture?

I seeded MEFs (passage 5), mitomycin C, or UV treated MEFs ( passage 6) in a 96-well plate at a density of 40, 000 cells/well, since I wanted the wells to be very confluent. I always see a lot of...

21 May 2014 467 4 View