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I want to check cell Monolayer Integrity on transwell. I have used the Lucifer Yellow Permeability Assay. According to Sigma-Aldrich company, a permeability of
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I want to check cell Monolayer Integrity on trans-well. is the Lucifer Yellow Permeability Assay is right method to be use? if yes, can you let me know the Cat no please?
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I have used anti-Calnexin antibody (1:200) from Santia Cruz (SC-23954) to do WB with human PTECs. I did not get the 90kDa band, but I did get the sharp band between 70-50 kDa and very weak band...
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