Xiang Ren

2 Questions 5 Answers 0 Followers

Questions related from Xiang Ren

Why does one PCR band after gene clean become four bands?

Hi, I run my PCR product on agarose gel, cut my 3kb target band and gene clean it. But when I run my gene clean product on agarose gel, apart from my 3kb target band, it also gives me another...

10 October 2014 8,105 7 View

Has anyone used the Gibson assembly reaction for 10kb vector?

I am planning to use Gibson assembly to put two pieces of DNA (both are ~1kb) into a 11.2 kb expression vector. This is my first time to do Gibson assembly, and I am not sure whether Gibson...

06 June 2013 5,289 7 View