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I have constructed a plasmid containing a R6K origin and kanamycin resistance marker and Tetracycline resistance marker. I then transformed this plasmid into a F' strain with a tetR marker on the...
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I have a question about using arabinose inducible promoter on plasmid inside e coli. If araC is present in the genome such as e coli, do I still need araC before pBAD promoter on the plasmid? I...
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I want to select for two plasmids in E. Coli. One contains a Chloramphenicol marker and one contains a spectinomycin marker. I selected transformants on LB + Cm (30μg/ml) + Sp (50μg/ml) plate....
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