Why I can not see any IL-6 production upon the stimulation of LPS on THP-1 cells using ELISA kit?

I have tried different concentrations of LPS (1, 10 ug/mL), on THP-1 cells differentiated by PMA (1 x 10^6 cells/mL, or 2 x 10^6 cells/mL), at different time points (3h, 6h and 24h). There is no...

08 August 2019 4,882 4 View