Xi Jiang

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Can amino acid change after protease cleavage?

Hello everyone, I am currently working with targeting proteins to yeast mitochondria. Our strategy is to use a mitochondrial targeting signal to transport our protein of interest to mitochondrial...

10 October 2019 3,782 5 View

Why is my his-tagged protein not detected in my western blots?

Dear all, I am having problems with my Western blots. I am doing transient expression of proteins in Nicotiana benthamiana using Agrobacterium tumefaciens. These two proteins are both his-tagged...

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