Xi Xiao Huang

2 Questions 2 Answers 0 Followers

Questions related from Xi Xiao Huang

Are cells able to preferentially excise/kick out your gene of interest, but not the antibiotic selection gene, from stable cells lines?

Hi all, I am trying to generate some stable cell lines expressing either the WT or mutant version of my gene of interest (GOI). My lentiviral construct consists of a BSD gene for blasticidin...

15 December 2018 9,241 3 View

How to block nylon membranes?

Hi all, I am trying to detect biotinylated DNA that has been transferred onto nylon membranes by chemiluminescent with biotin antibodies. However, I am getting very high background in my results,...

12 September 2018 5,682 5 View