Xander Wilcox

3 Questions 2 Answers 0 Followers

Questions related from Xander Wilcox

Cas variants that can form R-loops greater than 10-12 nucleotides?

I'm looking for a Cas variant that is able to create an R-loop greater than the 10-12 nucleotides that Cas9 forms. Ideally, I'd like to have a region of ssDNA between 20-30 nts, but anything...

09 April 2018 1,826 0 View

Insoluble primers mutagenesis primer stock?

I've noticed when thawed my 100 µM primer stock has precipitation that can't be resuspended with vortexing or slight heating. These primers also seem to give mutant plasmids with the WT sequence...

01 December 2017 1,147 4 View

Incorrect band size: Q5 Site-Directed Mutagenesis?

Hi all, I'm attempting to do a single nucleotide mutation in a 10.1 kb plasmid. I've attempted to do this with various quickchange primer pairs with no success so I designed primers for the Q5...

30 August 2017 1,786 4 View