Wynne Theresa

5 Questions 11 Answers 0 Followers

Questions related from Wynne Theresa

No Ethylene Detected in Acetylene Reduction Assay—Any Suggestions?

Hello, I have been trying to perform the acetylene reduction assay using pure bacterial cultures. Despite numerous attempts, including trials with positive control bacteria, I have not been able...

09 June 2025 2,132 0 View

Oxygen removal prior to Acetylene Reduction Assay (ARA)?

Hello. I am attempting to perform the acetylene reduction assay (ARA) to detect nitrogenase activity in bacteria known to fix nitrogen. However, the assay is not producing any detectable...

22 May 2025 2,961 0 View

What is the most suitable medium to use for ARA — liquid or semi-solid?

Hello. What is the most suitable medium to use for ARA — liquid or semi-solid? Does it depend on the bacterial strain? Any insights would be greatly appreciated. Thank you.

22 May 2025 2,825 0 View

What Is the Best Way to Store a Purified Enzyme for Stability?

Hello. I am working with a recombinant enzyme from a marine bacterium, attempting to overexpress it in E. coli and purify it using His-tag affinity chromatography. Since I cannot perform all...

01 April 2025 2,400 12 View

How to Improve Linearity in the Enzyme Stability Assays?

Hello, I am trying to examine the effects of pH and temperature on glycoside hydrolase enzyme stability using p-nitrophenyl-β-D-glucopyranoside as a substrate. This is the procedure that I...

01 April 2025 8,039 11 View