Can anyone suggest me possible reasons why I couldn't transform an insert using pUC18 plasmid?

Hi everyone! I have done  transformation using DH5alpha strain, pUC18 plasmid and Actinobacillus succinogenes fragment. fragment size=3748bp pUC18=2686bp XbaI and EcoRI restriction sites Though I...

03 July 2015 4,501 15 View