Wouter van der Westhuizen

2 Questions 6 Answers 0 Followers

Questions related from Wouter van der Westhuizen

What can I do to prevent my protein from precipitating on a Nickle IMAC column during denaturing conditions?

Hi I'm trying to purify an over-expressed membrane protein which I suspect is stuck in inclusion bodies. I extract the proteins using 8M Urea in a 50mM Tris-HCl (pH 7) buffer containing 20mM...

11 November 2015 4,136 7 View

Will size exclusion chromatography precipitate out my Triton X-100 solubilized hydrophobic 6xHIS-tagged protein onto the column?

I have purified my HIS-tagged protein with 1% Triton X-100 in the wash and elution buffers with a Nickel affinity column. The protein was over-expressed in E. coli and as it's a membrane protein,...

07 July 2015 3,231 5 View