Woojong Lee

6 Questions 5 Answers 0 Followers

Questions related from Woojong Lee

Cell counting using flow cytometry?

All- Is there a way to count lymphocytes from purified spleen using flow cytometry that will give comparable cell # from hemocytometer?

11 December 2017 7,193 5 View

FC block for intercellular staining for flow cytometry?

Dear all, I have a lot of non-specific bindings for one of the staining for intercellular staining in cells derived from mice. My 1' antibody is from rabbit. If I am doing just one color for ICCS,...

09 November 2016 8,183 3 View

96 wel culture-treated flat bottom plate - how to retrieve the cells?

Dear all- What would be the best way to retrieve the cells from 96 well culture-treated plates for staining and etc.? Thanks a lot!

11 October 2016 1,247 1 View

What is the best way for transfection in macrophages?

Dear all- What would be the best way to transfect macrophages/dendritic cells in vitro with plasmids for reporter assay or flourescent imaging with a good transfection efficiency without causing...

17 July 2016 7,438 9 View

Any suggestions for sticky protein crystals?

Dear all- My protein crystals stick to the bottom.. Is there any good suggestion to this? Thanks!

23 March 2016 3,217 2 View

DNA+Protein crystallization - what would be the quickest way to determine if my crystal is either DNA or protein crystals?

Hello All, I am currently trying to crystallize DNA+protein complex. What would be the quickest way to determine if my crystal is either DNA or protein crystals? Thanks a lot!

17 January 2016 7,857 1 View