Wonyong Lee

3 Questions 7 Answers 0 Followers

Questions related from Wonyong Lee

DO11.10 x Rag1 KO mice have very low CD4+ T cell number in spleen. Is this normal?

Hello, I have generated DO11.10 / Rag1 KO mice with deletion of gene of my interest. So it is double gene KO and TCR transgenic. I tried to isolate naive T cells from the mouse using FACS with...

10 October 2015 4,304 4 View

Which filter should I use for mouse lethal X-ray irradiation?

From previous post (link below), I found what I did wrong in my experiment. Fortunately there was a publication using the same X-ray irradiator and I followed their settings, which includes 0.5mm...

05 May 2015 6,690 1 View

Is skin lesion and low donor cell frequency in my bone marrow transfer model normal?

8-week-old C57BL/6 mice were irradiated from 7 to 10Gy with a new X-ray irradiator in an attempt to decide optimal X-ray dose for bone marrow chimera experiment. The goal was to find the dose of...

02 February 2015 4,147 6 View