Does anybody know a protocol to stimulate murine dendritic cells by JAK activation (e.g. IL-6 or IL-31) for testing JAK inhibitors?

I tried 48 h pre-incubation with rec. m. IFN gamma (should increase IL-31RA at least in human monocyte derived DC). Then stimulation with rec. m. IL-31, but I could not detect any TNF as described...

04 April 2017 8,483 2 View