Wisdom John Okoye

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Questions related from Wisdom John Okoye

Has anyone used a LightCycler Nano PCR machine to do a High Resolution Melting Thermal Shift Assay?

I am trying to do a high resolution melting thermal shift assay by applying a thermal shift assay control kit, to a LightCycler Nano Instrument from Roche. I cant seem to get anything off the...

06 September 2018 6,682 2 View

How do I deal with recombinant protein seperation after MBP Factor Xa cleavage digestion of recombinant fusion protein?

After purifying my recombination fusion protein sample using a Bio-rad Q-column chromatography, the resultant samples, were verified via SDS-PAGE analysis and stored in glycerol 20% concentration...

30 July 2018 5,974 3 View