Winde Jorissen

4 Questions 5 Answers 0 Followers

Questions related from Winde Jorissen

Modifications on pre-bèta versus larger forms of HDL?

Is it possible that freshly secreted (pre beta) HDL particles aren't (oxidatively) modified whereas larger (alpha 4, alpha 3,..) HDL particles are? In other words; is there a certain time needed...

01 September 2015 379 3 View

Having problems isolating LDL from pooled mice blood using density gradient ultracentrifugation

When isolating LDL from human blood samples we use density gradient ultracentrifugation according to the method of Proudfout et al. (blood drawn in EDTA coated tubes, spin off 10 min. at 3000rpm,...

28 July 2013 9,786 5 View

Why are non-physiological HDL concentrations (25-100µg/ml) used in macrophage culture experiments while concentrations in the blood are 40-60mg/dl?

In literature, many beneficial effects of HDL on macrophages have been described. Nonetheless, the HDL concentrations that are being used (25-100µg/ml) in almost every study don't seem to be...

07 March 2013 807 7 View

Lipoprotein isolation from human plasma samples - for further use in cell cultures

I would like to isolate the HDL2, HDL3, LDL and VLDL fractions from human plasma. After isolation I want to use the fractions seperately in cell cultures. I find a lot of different procedures to...

25 March 2012 1,793 15 View