I'm having trouble extracting total RNA from a culture of macrophages derived from monocytes infected with Leishmania infantum. Can someone help me?

I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...

13 May 2024 932 4 View