William Kim

4 Questions 14 Answers 0 Followers

Questions related from William Kim

Does anyone do CoCl2 induced hypoxia on retinal pigment epithelial?

I am making hypoxia situation in human pigment epithelial (RPE)using CoCl2. I read the some paper that the cobalt could make hypoxia situation and reduce the cell viability. however i used from...

01 January 2017 3,739 5 View

How disassemble or detach antibody and extracellular vesicle?

We will trying ti isolate marker positive extracellualr vesicle(EV), from pre-enriched EV sample using antibody conjugated bead. The used bead for antibody conjugation is aldehyde/sulfate or...

06 June 2016 2,971 3 View

Could GST tagged N-terminal binding with antibody?

I am trying to set up indirect ELISA using recombinant protein and antibody. The problem is the epitope of my antibody is "N-terminal". The most of recombinant protein I found is "tagged with GST...

01 January 2016 901 8 View

Can I use protein fragment (Not full length) as standard of ELISA?

Hi I am trying to developing indirect ELISA using antibody and recombinant protein (still finding). For protein stadndard I found some recombinant protein product from variety manufacture, but I...

01 January 2016 8,481 8 View