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Questions related from William Yeow
I am trying to grow some Sf9 cells but after incubation at 27 degrees and shaking at 125rpm for 2 days, they all die or at least majority of them are dead. I grew a 3mL culture (SF900II media) in...
19 September 2018 1,799 3 View
I cloned a gene into a vector with Kanamycin resistance and plate it on a Kanamycin plate and get many colonies. I then did colonyPCR followed by miniprep (I kept glycerol stock for those colonies...
01 December 2017 3,997 1 View
For Midipreps, can I use Potassium Acetate for neutralization instead of Soidum Acetate? Is there any reason why commercial kits use Sodium Acetate instead of Potassium Acetate? Also, why does...
27 June 2017 7,295 0 View
I am using Invitrogen Plasmid Midiprep kit and I am wondering which buffer and what components allow the DNA to bind to the filtration column. Usually it is the Binding buffer that does this but...
20 June 2017 5,545 3 View
