Why am I getting overestimated DNA concentration by A260 after phenol chloroform extraction?

I performed a standard PCIA extraction (2x PCIA, 1X chloroform) on my PCR samples to clean them up for use as an enzyme substrate. By A260, (nanodrop) I later found I have overestimated the [DNA]...

26 March 2020 1,136 6 View