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I need to build a multiple sequence alignment for my project, as I'm designing a PCR assay. I'm confused as to whether I should build the alignment using my PCR product or with the sequence I used...
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I am developing a PCR assay for E.coli and I have found its limit of detection for genomic DNA to be 0.0006 ng/ul, but I would like to convert this into CFU/ml count. Could I do a serial...
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