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Questions related from William K. Lim
I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...
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I thawed a tube of Calu-3 cells from ATCC, following product instructions. The flask I used was not coated with any substrate. I used EMEM with 10% FBS and placed it in the usual 37degC incubator...
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Has anyone used expired tissue culture plasticware such as flasks, dishes, tubes- and I particularly want to ask about serological pipettes. For how long after expiry did you find it safe to use?...
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Hi I seeded 96 well plates with 10,000 PC12 cells. I did this procedure 3 months ago using the same batch of 96 well plates (expiry only in 2017) and had seen cells growing OK in the wells - they...
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I need to measure the protein concentration of lipid raft isolated by density gradient centrifugation. Proteins are separated across a 25%, 20%, 15%, 10%, 5% and 0% Optiprep gradient. I collect...
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I need to culture PC12 cells without serum because in my experiments I want to see the effects of adding substances which are present in serum. Do you know of any commercially available...
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