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Questions related from William K. Lim
GppNHp and GTPgS are used to uncouple GPCR from G proteins. A lot of work is done by adding 10uM GppNHp to receptors expressed in cell membranes to study G protein activation leading to their...
17 July 2024 9,951 0 View
I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...
10 January 2024 7,553 7 View
I thawed a tube of Calu-3 cells from ATCC, following product instructions. The flask I used was not coated with any substrate. I used EMEM with 10% FBS and placed it in the usual 37degC incubator...
19 April 2022 4,447 3 View
Has anyone used expired tissue culture plasticware such as flasks, dishes, tubes- and I particularly want to ask about serological pipettes. For how long after expiry did you find it safe to use?...
10 April 2017 2,280 3 View
Hi I seeded 96 well plates with 10,000 PC12 cells. I did this procedure 3 months ago using the same batch of 96 well plates (expiry only in 2017) and had seen cells growing OK in the wells - they...
19 October 2016 4,666 6 View
I need to measure the protein concentration of lipid raft isolated by density gradient centrifugation. Proteins are separated across a 25%, 20%, 15%, 10%, 5% and 0% Optiprep gradient. I collect...
25 July 2016 9,051 3 View
I need to culture PC12 cells without serum because in my experiments I want to see the effects of adding substances which are present in serum. Do you know of any commercially available...
20 May 2016 8,285 2 View
