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Questions related from William Ford
I have been studying a dynamic protein and have selectively labelled a cysteine for 19F NMR. This cysteine has two peaks corresponding to a major and minor conformation. On a 500-MHz spectrometer,...
31 January 2023 9,609 11 View
I am working with a system in which my protein needs to have a disulfide reducer like tris(2-carboxyethyl)phosphine (TCEP) present, but the binding targets I'm studying have a nitroxide...
20 September 2022 7,106 2 View
In a 19F-NMR spectrum, the chemical shifts are usually negative. Therefore, does "upfield" become leftward movement towards zero, or does it still refer to rightward movement, but now becoming...
06 September 2022 1,920 6 View
I work with a protein that is normally well behaved, but I've recently developed the following intermittent issue: following cell lysis by sonication -- and multiple rounds of centrifugation at...
13 January 2022 1,735 4 View
Our lab is disposing of some old dansyl-substituted amino acids leftover from the previous lab that occupied our space. However, safety data sheets are few and far between, and the ones that I...
21 December 2020 3,916 0 View
I am curious if there exist any bioinformatics tools that can predict changes in secondary structure for proteins and/or nucleic acids. For instance, say a C-terminal loop on a protein reorganizes...
20 May 2020 2,271 8 View
I am looking at making an alanine-to-proline mutation, but I have never made one. What is known regarding the effects of this mutation? We are working with the hnRNP family.
07 April 2018 995 2 View
