William van Doorn

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Questions related from William van Doorn

How do I obtain the highest possible immunoprecipitation yields (possibly sacrificing specificity) in cell lysate samples?

For some months we have been trying to optimize several immunoprecipitations in order to achieve higher recoveries. So far our immunoprecipitations are very specific for our proteins of interest...

03 October 2017 5,903 2 View

Why do people employ Protein A in immunoprecipitation with Mouse IgG1 antibodies while affinity is very poor?

We all know the famous affinity tables of Protein A and G binding to different IgGs of several species. In fact, mouse IgG1 is known to bind (very) poorly to Protein A but posses a strong affinity...

19 April 2017 1,394 2 View