Willemijn Passtoors

3 Questions 4 Answers 0 Followers

Questions related from Willemijn Passtoors

Cell transfection - no overexpressed protein. Can someone tell me why?

I'm investigating some possible substrates and for this I need to over express proteins in mammalian cells and test some inhibitors on them. I'm doing two at the same time. One gene/protein is no...

09 September 2013 1,930 12 View

Updated: Difficulty measuring protein activity in mammalian cell lysate while western Blot is positive and no problems in e. coli - can anyone help?

I have a fluorescent assay to measure the activity of a certain membrane protein. When we use e. coli cell lysate, we see a clear difference in activity between e. coli with and without...

07 July 2013 9,759 10 View

What's the best/easiest way to scan all known human proteins for a certain sequence?

For my project we need to find (groups of) proteins with a certain sequence. What is the best and/or easiest way to screen all known human protein sequences for this particular sequence. I can use...

12 December 2012 4,561 1 View