Will Benman

4 Questions 1 Answers 0 Followers

Questions related from Will Benman

How to set up directed evolution to select for membrane recruitment?

Hello, I have a protein which binds the plasma membrane of cells. I'd like to do directed evolution to make its membrane binding affinity stronger, but I'm having trouble with figuring out the...

07 October 2020 2,452 1 View

Does anyone have a good source for designing cpf1/cas12a crRNA arrays?

Hello, I'm trying to design an array of 4 crRNAs for use with cpf1/cas12a both the As and Lb versions. I've found the scaffold and PAM sequence from digging through a few papers, but I can't find...

13 September 2020 4,161 2 View

Getting yeast colonies after transformation, but colony PCR is negative?

I am trying to tag the C terminus of endogenous genes CDC15 and 24 (both genes neccessary for growth) with mScarlet. I transformed a PCR product with the following sequence: 40bp...

20 October 2018 5,277 2 View

Where can I find the nucleotide sequence trp1delta63 and its promoter?

I am attempting to transform into a strain containing the trp1delta63 mutation and I am encountering problems. I suspect this is because of the sequence of the trp1delta63 promoter. However, I...

07 September 2018 3,824 2 View