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I am trying to purify a bacterial cytosolic protein from E.coli as heterologous host. It has a C-terminal 6xhis-tag. I have previously purified reasonable yields on NiNTA or Cobalt columns, but...
03 March 2018 9,234 10 View
Recently, we observed with one particular construct consistently single bp deletions in Gibson assemblies. The 1bp deletions almost exclusively occur in the areas of the primers used to amplify...
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We are frequently using plasmids with a certain backbone. Due to circumstances, some of these were maintained in Ecoli MC1061 and others in DH5a. Recently, we experienced problems with digests...
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I am trying to find out if there are any reasonable guestimates out there on the amount of protein in a total bacterial lysate from a certain amount of cells. All I could find so far is estimates...
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