Whitney L. Simon

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Questions related from Whitney L. Simon

Has anyone compared Miltenyi's and Stem Cell Technologies' MACS reagents and magnets for CNS tissue?

I've had good luck with Miltenyi's column-based MACS isolation of glial cells, but I'm wondering how Stem Cell Technologies' EasySep method compares since it does not involve columns or the force...

16 October 2023 6,800 0 View

Should PBS with or without Ca++ and Mg++ for perfusion and primary cell isolation before and after (papain) digestion (for CNS tissue specifically)?

This question is in regards to CNS tissue/cells specifically. I know Ca++ and Mg++ should be avoided during dissociation when using EDTA and trypsin. I use a papain dissociation but still avoid...

16 October 2023 1,893 0 View