OD at 280nm (A280) for a protein comes from absorbance of Trp, Tyr and Cystine. But, what about protein differences in glycosylation?

Most of the Absorbance or OD at 280nm (A280) for a protein comes from absorbance of Trp, Tyr and Cystine. But, what fraction of A280 units could/might be expected to come from protein differences...

11 November 2012 4,806 7 View