Wesam Gawad

3 Questions 2 Answers 0 Followers

Questions related from Wesam Gawad

Protocol for double digestion with thermo scientific fast digest enzyme?

optimal reaction set up for double digest reaction with XhoI and EcoRI using my PCR product about 1600 bp and vector Pet 22 about 5500bp

05 April 2019 3,628 4 View

What is the best range of melting temp for primers?

I have designed a few sets of primer with a restriction site, most of them with tm 69-74 is this okay for PCR reaction

04 December 2018 1,016 6 View

How to set up a dry ice ethanol bath?

Dry Ice / Ethanol baths are used to rapidly cool solutions to below freezing temperatures. Dry Ice / Ethanol baths can be used to freeze tissue sections for histology or to rapidly cool Ethanol...

02 October 2018 6,611 2 View