Can I build into at 1.0-1.5 sigma into a Fo-Fc map?

I am using Fo-Fc, 2Fo-Fc, and a DM maps to build my protein in COOT at ~3A resolution. I know the gene sequence of my protein. What is the ideal contour level(sigma) setting for building into a...

26 April 2012 9,854 4 View