Why is RT-qPCR Cq Value of internal reference gene(GAPDH) too high?

I used Trizol method to extract cellular RNA, and A260/A280 was always around 2.1. The RT reagent of TAKARA and the qPCR reagent of TAKARA were used. The experimental steps were all in accordance...

23 February 2021 2,298 5 View