Wenxiu Ning

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Questions related from Wenxiu Ning

Ligation vector with DNA fragment failed, and don't know why. Vector ~8.2kb; DNA fragment ~1kb. Can anyone help?

I want to ligate x-vector with one DNA fragment. The vector was digested using BspE1 and Bgl II, and the DNA fragment was digested using BspE1 and BamhI, I used TAKARA solution I to ligate them,...

09 September 2014 8,112 16 View

Does anyone have the protocol for establishing the stable expression cell line?

The normal cell still lives when G418 is added, what is the lowest concentration of G418 to kill the normal cells but not the transfected cells? How can cells be made to express proteins at the...

01 January 2013 5,734 2 View