Wen An Pan

4 Questions 6 Answers 0 Followers

Questions related from Wen An Pan

Can a good gRNA efficiency in one cell be necessarily applied to other cells?

Hi everyone, If I have 3 gRNAs, will you suggest to screen in 293 first to identify the "best" one and then only use that one to generate KO in our interest cells? Or the result in one cell line...

12 December 2016 9,765 3 View

Hi. Does anyone have experience of using cas9-GFP for FACS sorting and screening crispr KO clone? Any suggestion for the starting cell numbers?

Hi, I am trying to use px458 plasmid (Cas9-2A-GFP) to generate crispr KO cells and wonder if there's anyone having the experience could suggest the starting number of cells for screening. If the...

08 August 2016 8,228 2 View

If a plasmid contains more than one ATG and stop codon in front of the gene of interest, will they transcribe/translate unexpected RNA/proteins?

Hi, I found there are 1 ATG and 1 stop codon in front of our interested gene. Although we can still induce protein expression by Doxicycline in cells(in pSLIK-vector), is it possible that other...

08 August 2016 2,619 7 View

Luciferase plasmid for bioluminescence imaging in mouse tumor model?

Hi, For bioluminescence imaging of tumor growth in mouse, is it better to first generate the cell line expressing luciferase by retro/lentivirus, and then transduce the gene of our interest (GOI)...

06 June 2016 318 0 View