Wen An Pan

3 Questions 5 Answers 0 Followers

Questions related from Wen An Pan

Does someone have experience in generating crispr-KO luminal breast cancer cell line e.g. MCF7/T47D?

Hi, Has someone successfully generated crispr-knockout luminal breast cancer e.g. MCF7/T47D/BT474? I tried to transfect MCF7 and T47D with px458 (gRNA & cas9-GFP) plasmid, suspended cells in...

12 January 2017 3,403 2 View

Can a good gRNA efficiency in one cell be necessarily applied to other cells?

Hi everyone, If I have 3 gRNAs, will you suggest to screen in 293 first to identify the "best" one and then only use that one to generate KO in our interest cells? Or the result in one cell line...

22 December 2016 8,754 3 View

If a plasmid contains more than one ATG and stop codon in front of the gene of interest, will they transcribe/translate unexpected RNA/proteins?

Hi, I found there are 1 ATG and 1 stop codon in front of our interested gene. Although we can still induce protein expression by Doxicycline in cells(in pSLIK-vector), is it possible that other...

08 August 2016 2,923 6 View