Weiyan Jia

4 Questions 10 Answers 0 Followers

Questions related from Weiyan Jia

How much chromatin will go to soluble fraction after sonication in ChIP assay?

I noticed people use different lysis buffer to extract chromatin in ChIP assay(cross-linking). Do salt and detergent help to dissolve chromatin? Some paper pointed out mononucleosome still show...

02 February 2018 7,423 2 View

How to image Mitotic phases cells?

Hi everyone, I want to image mitotic phases cells, anyone can give me some suggestions? Do I need to synchronize cells to enrich more mitotic cells? I tried to synchronize cells to G2/M using...

12 December 2017 9,752 3 View

After selection of retrovirus infected cells, only few of the cells show target protein expression, any ideas?

I used retrovirus packaged my gene(7.5kb) tagged with Myc and HA tags, and then infected U2OS cells. After selection with antibiotic, I can not detect the protein using WB. IF assay showed, only a...

12 December 2016 3,849 5 View

Why negative control plates got a lot of colonies using Single-step digestion-ligation cloning of CRISPR plasmid?

Based on Fengzhang lab protocol provided on their lab website,I performed the one step digestion and ligation, I got a lot of colonies on negative control plate. I am wondering the cut sequence...

10 October 2016 3,526 4 View