2 Questions 3 Answers 0 Followers
Questions related from Weiliang Zuo
I have expressed the kinase domain of my protein in E.coli fused with GST tag, and have purified it. But it seemed to have no activity in vitro. My protein is about 40KD, does the 26KD GST tag...
12 December 2013 5,560 1 View
The kinase domain was expressed in E.coli fused with GST, and purified. I conducted kinase reaction with ATP, and tried to use phostag to separate the mock (no ATP added) and treated sample, but...
11 November 2013 7,460 1 View