Use a T7 exonuclease to generate a 20nt ssDNA overhang after PCR?

Hi, I am generating a ssDNA overhang from my PCR amplification. The length of the PCR product is ~160bp. In the forward primer, the first 20nt is regular DNA and the next 6 nt is pt modified. In...

3 weeks ago 5,907 0 View