Why is Col8A1 ~70kDa under reducing conditions (DTT) on SDS-PAGE?

Recombinant collagen 8A1(Col8A1) is expressed and purified from HEK293 cells. It runs at ~250kDa under non-reducing condition on SDS-PAGE, but why is it dissociating into a single chain (70kDa) on...

20 October 2020 6,024 2 View